Cov txheej txheem tsim cov poov xab cell phab ntsa

Poov xab cell phab ntsa cov khoom yog cov khoom tau los ntawm centrifuging poov hlwb tom qab autolysis, exogenous enzyme hydrolysis, mechanical fragmentation, thiab lwm yam kev kho mob kom tau cell phab ntsa. Cov khoom kawg yog lub teeb daj rau daj xim av hmoov tsis muaj ntxhiab tsw.
Cov txheej txheem dav dav
Cov poov xab puree → Dilute nrog dej (ntxuav kom txo qhov kev tshuaj ntsuam xyuas) → Lim thiab tshem tawm cov impurities → Txiav thiab cais (ntoo tawm supernatant) → Tshem tawm iab thiab tsw (ntxiv dej thiab ntsev kom tau cov kua ntshiab ntshiab) → Poov autolysis (tswj Cov xwm txheej: kub, sijhawm, pH tus nqi, thiab lwm yam) → Phab ntsa tawg cov poov xab enzymatic hydrolysis (tswj pH tus nqi, kub, lub sij hawm, enzyme ntau npaum li cas) → Sterilize thiab inactivate enzymes (tswj kub thiab lub sij hawm) → Centrifuge cais (sib cais poov cell phab ntsa thiab poov xab extract) → Concentrate → Qhuav → poov xab cell phab ntsa tiav khoom.
kev cuam tshuam
Ib qho yog cov txheej txheem autolysis. Nws tus kheej dissolving kub, pH, tshuaj tiv thaiv lub sij hawm, cosolvent muaj pes tsawg leeg, ntxiv npaum li cas, thiab lwm yam. Qhov thib ob yog cov txheej txheem enzymatic hydrolysis. Enzyme npaj xaiv, enzymatic hydrolysis tsis (pH nqi, kub, sij hawm, enzyme ntau npaum), thiab lwm yam. Qhov thib peb yog tshuaj tsuag kom qhuav. Lub sij hawm ziab, kub, thiab lwm yam tuaj yeem cuam tshuam cov dej noo thiab xim ntawm cov khoom tiav.
Duan Shenglin et al. pom tias qhov tsim nyog autolysis tej yam kev mob yog: autolysis kub ntawm {{0}} degree, pH ntawm 5. {17}}.8% concentration NaCl thiab 200 mg/LVB2 raws li poov tshuaj autolysis promoters, nrog ib tug solid-kua piv ntawm 1:8-10. Raws li cov xwm txheej no, tus nqi rho tawm tuaj yeem ncav cuag li 70%. Tom qab kev tsom xam, qhov tsim nyog rho tawm tej yam kev mob rau cov poov xab cell phab ntsa khoom yog: kub ntawm 60 degree, solid-kua piv ntawm 1:10, protease ntxiv ntawm 0.02-0.03%, thiab NaOH concentration ntawm 2%.